EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
    1.2.7.1 | 2-oxo-4-methyl-thio-butyrate + CoA + 2 methyl viologen |
enzyme Ape2126/2128, 37% of the activity compared to pyruvate |
Aeropyrum pernix |
? |
- |
? |
| 1.2.7.1 | 2-oxoadipate + CoA + 2 methyl viologen |
enzyme Ape2126/2128, 37% of the activity compared to pyruvate |
Aeropyrum pernix |
? |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized benzyl viologen |
- |
Methanosarcina barkeri |
propanoyl-CoA + CO2 + 2 reduced benzyl viologen |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized benzyl viologen |
coenzyme F420 is not reduced by the purified enzyme |
Methanosarcina barkeri |
propanoyl-CoA + CO2 + 2 reduced benzyl viologen |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized benzyl viologen |
coenzyme F420 is not reduced by the purified enzyme |
Methanosarcina barkeri DSM 804 |
propanoyl-CoA + CO2 + 2 reduced benzyl viologen |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized benzyl viologen |
- |
Methanosarcina barkeri DSM 804 |
propanoyl-CoA + CO2 + 2 reduced benzyl viologen |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + 2 oxidized methyl viologen |
enzyme Ape2126/2128, 98% of the activity compared to pyruvate |
Aeropyrum pernix |
propanoyl-CoA + CO2 + 2 reduced methyl viologen |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + oxidized methyl viologen |
the enzyme reacts with 2-oxobutyrate and oxaloacetate at 38% activity relative to the activity against pyruvate |
Hydrogenobacter thermophilus |
propanoyl-CoA + CO2 + reduced methyl viologen |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + oxidized methyl viologen |
the enzyme reacts with oxaloacetate at 4.8 activity relative to the activity against pyruvate |
Giardia intestinalis |
propanoyl-CoA + CO2 + reduced methyl viologen |
- |
? |
| 1.2.7.1 | 2-oxobutyrate + CoA + oxidized methyl viologen |
the enzyme has a broad substrate specificity. In the presence of CoA, it oxidizes pyruvate, oxaloacetate, 2-oxobutyrate, and indol-3-pyruvate with specific activities of 7.4, 6.5, 3.6, and 0.3 U/mg, respectively. The enzyme reduces clostridial rubredoxin, clostridial and spinach ferredoxin, cytochrome c, FMN, and FAD. No activity is detected with NAD+, NADP+, and vitamin K1. The catalytic efficiencies or kcat/Km values for FAD and FMN are calculated to be 2400032000/min * M, which are about two orders of magnitude lower than observed for the likely physiological electron carriers of other pyruvate oxidoreductases. Therefore, it is unlikely that flavins are the physiological electron carrier of the methanococcal pyruvate oxidoreductase |
Methanococcus maripaludis |
propanoyl-CoA + CO2 + reduced methyl viologen |
- |
? |
