EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.2.1.B25 | a long-chain fatty acyl-CoA + NADPH + H+ |
recombinant protein nearly exclusively produces C18:0 fatty alcohol |
Arabidopsis thaliana |
a long-chain aldehyde + CoA + NADP+ |
aldehyde intermediate is immediately reduced into the corresponding alcohol, chain lengths of primary alcohols produced by FAR heterologous expression in yeast are consistent with the chain lengths of primary alcohols found in Arabidopsis suberin |
? |
1.2.1.B25 | a long-chain fatty acyl-CoA + NADPH + H+ |
recombinant protein produces C18:0 and C20:0 fatty alcohols |
Arabidopsis thaliana |
a long-chain aldehyde + CoA + NADP+ |
aldehyde intermediate is immediately reduced into the corresponding alcohol, chain lengths of primary alcohols produced by FAR heterologous expression in yeast are consistent with the chain lengths of primary alcohols found in Arabidopsis suberin |
? |
1.2.1.B25 | a long-chain fatty acyl-CoA + NADPH + H+ |
recombinant protein produces C18:0 and C22:0 fatty alcohols, C16:0-OH, C20:0-OH, and C24:0-OH also detectable |
Arabidopsis thaliana |
a long-chain aldehyde + CoA + NADP+ |
aldehyde intermediate is immediately reduced into the corresponding alcohol, chain lengths of primary alcohols produced by FAR heterologous expression in yeast are consistent with the chain lengths of primary alcohols found in Arabidopsis suberin |
? |
1.2.1.B25 | dodecanoyl-CoA + NADPH |
low activity |
Synechococcus elongatus |
dodecanal + CoA + NADP+ |
- |
? |
1.2.1.B25 | dodecanoyl-CoA + NADPH |
low activity |
Synechococcus elongatus PCC7942 |
dodecanal + CoA + NADP+ |
- |
? |
1.2.1.B25 | eicosanoyl-CoA + NADPH |
- |
Synechococcus elongatus |
eicosanal + CoA + NADP+ |
- |
? |
1.2.1.B25 | hexadecanoyl-CoA + NADPH + H+ |
low activity |
Synechococcus elongatus |
hexadecanal + CoA + NADP+ |
- |
? |
1.2.1.B25 | hexadecanoyl-CoA + NADPH + H+ |
low activity |
Synechococcus elongatus PCC7942 |
hexadecanal + CoA + NADP+ |
- |
? |
1.2.1.B25 | more |
detailed analysis of FAR6 gene promotor |
Arabidopsis thaliana |
? |
- |
? |
1.2.1.B25 | more |
the enzyme cACR is specific for NADPH and specifically catalyzes the reduction of fatty acyl-CoA esters to the corresponding aldehydes, rather than alcohols. Stearoyl-CoA is the most effective substrate, being reduced more rapidly than either longer or shorter chain acyl-CoAs. The enzyme is acylated on incubation with stearoyl-CoA, suggesting that the reduction occurs through an enzyme-thioester intermediate, cf. EC 1.2.1.50, formation of an acyl thioester on an active site cysteinyl residue as an intermediate in the mechanism of reduction. No activity with octanoyl-CoA |
Synechococcus elongatus |
? |
- |
? |