EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.18.1.5 | 2 ferricytochrome c + NADH |
- |
Pseudomonas putida |
2 ferrocytochrome c + NAD+ + H+ |
- |
? |
| 1.18.1.5 | Fe(CN)62- + NAD+ |
- |
Pseudomonas putida |
Fe(CN)63- + NADH + H+ |
- |
? |
| 1.18.1.5 | more |
bulky side chains of Tyr33, Arg66, and Trp106 prevent tight binding of oxidized Pdx and facilitate dissociation of the reduced iron-sulfur protein from Pdr. Transfer of an electron from FAD to [2Fe-2S] can occur with various orientations between the cofactors through multiple electron transfer pathways that do not involve Trp106 but are likely to include Asp38 and Cys39 |
Pseudomonas putida |
? |
- |
? |
| 1.18.1.5 | more |
reductase is a two-eleetron acceptor with no stable semiquinone intermediate being formed either during reduction or air reoxidation |
Pseudomonas putida |
? |
- |
? |
| 1.18.1.5 | more |
the midpoint oxidation-reduction potential of PdR is -369 mV at pH 7.6, which is more negative than the pyridine nucleotide NADH/NAD+. The midpoint potential is a hyperbolic function of increasing NAD+ concentration, such that at concentrations of pyridine nucleotide typically found in an intracellular environment, the midpoint potential would be -230 mV, thereby providing the thermodynamically favorable redox equilibria that enables electron transfer from NADH, with thermodynamic control of electron transfer. The PdRox:NAD+ complex is about 5 orders of magnitude weaker than PdRrd:NAD+ binding. These results support a compulsory ordered pathway to describe the electron-transfer processes |
Pseudomonas putida |
? |
- |
? |
| 1.18.1.5 | more |
wild-type and His6 Pdr are able to function as NAD(H)-dependent dithiol/disulfide oxidoreductases catalyzing both forward and reverse reactions, NAD+-dependent oxidation of thiols, and NADH-dependent reduction of disulfides. This function of the flavoprotein can be dissociated from electron transfer to putidaredoxin |
Pseudomonas putida |
? |
- |
? |
| 1.18.1.5 | NADH + H+ + Fe(CN)63- |
- |
Pseudomonas putida |
NAD+ + Fe(CN)62- |
- |
? |
| 1.18.1.5 | NADH + H+ + oxidized 2,6-dichlorophenolindophenol |
- |
Pseudomonas putida |
NAD+ + reduced 2,6-dichlorophenolindophenol |
- |
? |
| 1.18.1.5 | putidaredoxin + NADH |
- |
Pseudomonas putida |
? |
- |
? |
| 1.18.1.5 | reduced 2,6-dichlorophenolindophenol + NAD+ |
- |
Pseudomonas putida |
oxidized 2,6-dichlorophenolindophenol + NADH + H+ |
- |
? |
