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EC Number	Substrates	Commentary Substrates	Organism	Products	Commentary (Products)	Reversibility
1.14.99.69	2-chloro-N6-isopentenyladenosine + reduced acceptor + O2	-	Salmonella enterica subsp. enterica serovar Typhimurium	2-chloro-N6-((E)-4-hydroxyisopentenyl)-adenosine + acceptor + H2O	-	?
1.14.99.69	i6A + reduced acceptor + O2	i.e. free nucleoside	Salmonella enterica subsp. enterica serovar Typhimurium	io6A + acceptor + H2O	-	?
1.14.99.69	i6A-ACSLTrp + reduced acceptor + O2	i.e. isopentenylated anticodon stem loop (ACSL) region of tRNATrp	Salmonella enterica subsp. enterica serovar Typhimurium	io6A-ACSL-Trp + acceptor + H2O	-	?
1.14.99.69	more	enzyme additionally shows catalase activity. Formation of 1.1 mol of O2 is observed upon addition of 2 mol H2O2	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	-
1.14.99.69	more	hydroxylation of the terminal isopentenyl-C4-position is observed with more than 97% E-stereoselectivity. No other nonspecific hydroxylation products are observed in enzymatic assays. The initial rate of MiaE hydroxylation is highly influenced by the substituent at the C2-position of the nucleoside base. MiaE exhibits more than 3fold enhancement for the hydroxylation of the free ms2i6A nucleoside relative to i6A	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	-
1.14.99.69	more	steady-state product formation retains more than 98%stoichiometric E stereoselectivity	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	-
1.14.99.69	ms2i6A + reduced acceptor + O2	ms2i6A i.e. N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine37 in tRNA	Salmonella enterica subsp. enterica serovar Typhimurium	ms2io6A + acceptor + H2O	-	?
1.14.99.69	N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine37 in tRNA + reduced acceptor + O2	-	Salmonella enterica subsp. enterica serovar Typhimurium	N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O	-	?
1.14.99.69	N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine37 in tRNA + reduced acceptor + O2	-	Pseudomonas putida	N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O	-	?
1.14.99.69	N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine37 in tRNA + reduced acceptor + O2	-	Pseudomonas putida DSM6125	N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O	-	?

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Release 2023.1 (January 2023)