EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  1.14.99.46 | more |
the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate |
Escherichia coli |
? |
- |
? |
| 1.14.99.46 | more |
(Z)-3-ureidoacrylic peracid decomposes nonenzymatically to form primarily (Z)-3-ureidoacrylic acid and uracil, half life of (Z)-3-ureidoacrylic peracid is 3 hours at pH 10.0 at 25°C and much less at lower pH |
Escherichia coli |
? |
- |
? |
| 1.14.99.46 | more |
(Z)-3-ureidoacrylic peracid is not stable and decomposes nonenzymatically to give (Z)-3-ureidoacrylate |
Escherichia coli |
? |
- |
? |
| 1.14.99.46 | thymine + FMNH2 + O2 |
- |
Escherichia coli |
(Z)-2-methylureidoacrylate peracid + FMN |
- |
? |
| 1.14.99.46 | uracil + FMNH2 + O2 |
- |
Escherichia coli |
(Z)-3-ureidoacrylate peracid + FMN |
- |
? |
| 1.14.99.46 | uracil + FMNH2 + O2 |
RutA directly cleaves the uracil ring between N-3 and C-4 to yield ureidoacrylate |
Escherichia coli |
(Z)-3-ureidoacrylate peracid + FMN |
product analysis by both NMR spectroscopy and mass spectrometry, overview |
? |
