EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.15.15 | (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 |
the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver |
Homo sapiens |
? |
- |
? |
1.14.15.15 | (24S)-3beta-hydroxy-24-methyl-5alpha-cholesta-8(14),22-dien-15-one + reduced adrenodoxin + H+ + O2 |
28-hydroxylation by CYP27A1, enzyme activity assay in presence of adrenodoxin, adrenodoxin reductase and NADPH |
Homo sapiens |
? |
massspectrometric product analysis, 2 products, overview |
? |
1.14.15.15 | (25R)-5beta-cholestane-3alpha,7alpha,12alpha,27-tetraol + reduced adrenodoxin + O2 |
- |
Oryctolagus cuniculus |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.15 | 1alpha-hydroxyvitamin D3 + reduced adrenodoxin + O2 |
25-hydroxylation |
Rattus norvegicus |
1alpha,25-dihydroxyvitamin D3 + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.15 | 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al + reduced adrenodoxin + O2 |
- |
Oryctolagus cuniculus |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.15 | 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 |
the substrate is a potential drug for lowering cholesterol in the treatment and/or prevention of coronary artery disease, but it is rapidly metabolized in the liver |
Homo sapiens |
? |
- |
? |
1.14.15.15 | 3beta-hydroxy-5alpha-cholest-8(14)-en-15-one + reduced adrenodoxin + H+ + O2 |
28-hydroxylation by CYP27A1, enzyme activity assay in presence of adrenodoxin, adrenodoxin reductase and NADPH |
Homo sapiens |
? |
massspectrometric product analysis, 2 products, overview |
? |
1.14.15.15 | 5-cholestene-3beta,7alpha-diol + reduced adrenodoxin + O2 |
- |
Oryctolagus cuniculus |
5-cholestene-3alpha,7alpha,26-triol + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.15 | 5beta-cholestane-3alpha,7alpha,12alpha,26-tetraol + reduced adrenodoxin + O2 |
- |
Homo sapiens |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.15 | 5beta-cholestane-3alpha,7alpha,12alpha,26-tetraol + reduced adrenodoxin + O2 |
- |
Rattus norvegicus |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al + oxidized adrenodoxin + H2O |
- |
? |