EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.13.44 | 2,2'-dihydroxybiphenyl + NADH + O2 |
- |
Pseudomonas nitroreducens |
2,2',3-trihydroxybiphenyl + NAD+ + H2O |
- |
? |
1.14.13.44 | 2,2'-dihydroxybiphenyl + NADPH + O2 |
- |
Pseudomonas nitroreducens |
2,2',3-trihydroxybiphenyl + NADP+ + H2O |
- |
? |
1.14.13.44 | 2,3-dihydroxybiphenyl + NADH + H+ + O2 |
- |
Pseudomonas nitroreducens |
? |
- |
? |
1.14.13.44 | 2,5-dihydroxybiphenyl + NADH + O2 |
- |
Pseudomonas nitroreducens |
2,3,5-trihydroxybiphenyl + NAD+ + H2O |
- |
? |
1.14.13.44 | 2-ethylphenol + NADH + O2 |
- |
Pseudomonas nitroreducens |
1,2-dihydroxy-3-ethylbenzene + NAD+ + H2O |
- |
? |
1.14.13.44 | 2-hydroxybiphenyl + NADH + H+ + O2 |
- |
Pseudomonas nitroreducens |
2,3-dihydroxybiphenyl + NAD+ + H2O |
- |
? |
1.14.13.44 | 2-hydroxybiphenyl + NADH + H+ + O2 |
it is suggested that Trp225, which is located in the active site, facilitates proper substrate entrance into the binding pocket |
Pseudomonas nitroreducens |
2,3-dihydroxybiphenyl + NAD+ + H2O |
- |
? |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 |
- |
Pseudomonas nitroreducens |
2,3-dihydroxybiphenyl + NAD+ + H2O |
- |
? |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 |
ternary complex mechanism in which the aromatic substrate has strict control in both the reductive and oxidative half-reaction in a way that reactions leading to substrate hydroxylation are favored over those leading to the futile formation of hydrogen peroxide. NAD+ release from the reduced enzyme-substrate complex is the slowest step in catalysis |
Pseudomonas nitroreducens |
2,3-dihydroxybiphenyl + NAD+ + H2O |
- |
? |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 |
the activity of the mutant enzyme HbpAind is six times lower than that of the wild-type enzyme |
Pseudomonas nitroreducens |
2,3-dihydroxybiphenyl + NAD+ + H2O |
- |
? |