EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.14.13.180 | 11-deoxy-beta-L-rhodomycinone + NADPH + H+ + O2 |
11-deoxy-L-rhodomycinone is a better substrate than aklavinone |
Streptomyces purpurascens |
beta-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADH + H+ + O2 |
the enzyme participates in the biosynthesis of daunorubicin, doxorubicin and rhodomycins |
Streptomyces purpurascens |
epsilon-rhodomycinone + NAD+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADPH + H+ + O2 |
- |
Streptomyces purpurascens |
epsilon-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADPH + H+ + O2 |
- |
Streptomyces peucetius |
epsilon-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADPH + H+ + O2 |
the enzyme is involved in the biosynthesis of the anthracycline antibiotic daunorubicin/doxorubicin pathway. During the biosynthesis of L-rhodomycins, RdmE first modifies aklavinone at C-11, and epsilon-rhodomycinone or aklavinone is subsequently glycosylated |
Streptomyces purpurascens |
epsilon-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADPH + H+ + O2 |
about 60% compared to the activity with 11-deoxy-L-rhodomycinone |
Streptomyces purpurascens |
epsilon-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADPH + H+ + O2 |
modifications of the aklavinone molecule can influence the efficiency of the hydroxylation carried out by DnrF. While molecules modified in positions C-10 and C-13 are hydroxylated as efficiently as aklavinone (11-deoxycarminomycinone conversion), molecules 14-hydroxylated or 4-methylated are very poor substrates (11-deoxy-4-demethyladriamycinone and 11-deoxydaunomycinone conversion) |
Streptomyces peucetius |
epsilon-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADPH + H+ + O2 |
- |
Streptomyces peucetius ATCC 29050 |
epsilon-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | aklavinone + NADPH + H+ + O2 |
modifications of the aklavinone molecule can influence the efficiency of the hydroxylation carried out by DnrF. While molecules modified in positions C-10 and C-13 are hydroxylated as efficiently as aklavinone (11-deoxycarminomycinone conversion), molecules 14-hydroxylated or 4-methylated are very poor substrates (11-deoxy-4-demethyladriamycinone and 11-deoxydaunomycinone conversion) |
Streptomyces peucetius ATCC 29050 |
epsilon-rhodomycinone + NADP+ + H2O |
- |
? |
| 1.14.13.180 | C-glucosyl flavokermesic acid + NADPH + H+ + O2 |
- |
Streptomyces peucetius |
carminic acid + NADP+ + H2O |
- |
? |
