EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.13.11.57 | 3,4,5-trihydroxybenzoate + O2 |
DesB has strict substrate specificity for 3,4,5-trihydroxybenzoate (gallate). Biochemical and structural study show the substrate recognition and catalytic mechanisms of extradiol dioxygenase DesB. Mutational analysis reveals that His124 and His192 in the active site are essential to the catalytic reaction of the extradiol dioxygenase DesB. His124, which interacts with OH(4) of the bound gallate, seems to contribute to proper positioning of the substrate in the active site. His192, which is located close to OH (3) of the gallate, is likely to serve as the catalytic base. Glu377' interacts with OH(5) of the gallate and seems to play a critical role in the substrate specificity |
Sphingobium sp. SYK-6 |
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate |
- |
? |
1.13.11.57 | gallate + O2 |
gallate is also namend gallic acid or 3,4,5-trihydroxybenzoic acid |
Pseudomonas putida |
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate |
- |
? |
1.13.11.57 | gallate + O2 |
gallate is also namend gallic acid or 3,4,5-trihydroxybenzoic acid |
Pseudomonas putida KT 2240 |
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate |
- |
? |
1.13.11.57 | gallate + O2 |
- |
Sphingobium sp. |
4-oxalomesaconate |
- |
? |
1.13.11.57 | gallate + O2 |
- |
Sphingomonas paucimobilis |
4-oxalomesaconate |
- |
? |
1.13.11.57 | gallate + O2 |
the substrate specificity is very restricted toward gallate |
Pseudomonas putida |
4-oxalomesaconate |
- |
? |
1.13.11.57 | gallate + O2 |
- |
Sphingomonas paucimobilis SYK-6 |
4-oxalomesaconate |
- |
? |
1.13.11.57 | gallate + O2 |
the substrate specificity is very restricted toward gallate |
Pseudomonas putida KT 2240 |
4-oxalomesaconate |
- |
? |
1.13.11.57 | gallate + O2 |
- |
Sphingobium sp. SYK-6 |
(2E,4E)-4-(carboxymethylidene)-2-hydroxypent-2-enedioate |
- |
? |
1.13.11.57 | more |
no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate |
Sphingomonas paucimobilis |
? |
- |
? |