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Results 1 - 10 of 103 > >>
EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
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joins single-stranded DNA molecules much less efficiently. Many of them approximate a well-defined consensus sequence
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RNA-stimulated exchange reaction between ATP and diphosphate
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efficient ligation of single-stranded DNAs and RNAs
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synthesizes circular hammerhead ribozymes from oligonucleotides, small RNA molecules resistant to circuarization by other means can be efficiently circularized on a preparative scale using a suitable DNA template
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no activity with alendronate nor pamidronate
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the group I intron from cyanobacterium Anabaena sp. catalyzes phosphodiester bond formation using a triphosphate on the 5'-terminal nucleotide, much like protein polymerases and engineered ribozymes. In the process, this ribozyme forms a unique circular RNA that incorporates the exogenous guanosine cofactor added during self-splicing
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enzyme does not discriminate between RNA and DNA for phosphodiester bond formation
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intramolecular ligation of 5'-PO4 single-strand RNA to form a covalently closed circular RNA molecule through ligase-adenylylate and RNA-adenylylate intermediates AppRNA. At the optimal temperature of 65°C, AppRNA is predominantly ligated to a circular product. At 35°C, phosphodiester bond formation is suppressed and the majority of the AppRNA is deadenylylated
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T4 RNA ligase possesses nick-joining activity in RNA/DNA hybrids against junctional matches/mismatches complementary to the RNA strand as a ligation template. When a junctional mismatch is present at the 30-terminal of RNA probe, the nick-joining activity of T4Rnl2 is inhibited
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the RNA ligase has a a strict requirement for RNA substrates with a 2'-phosphate terminus for the ligation of RNAs with 5'-phosphate and 3'-hydroxyl ends. RNA ligase forms a 2'-phosphomonoester-3',5'-phosphodiester junction
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Results 1 - 10 of 103 > >>