EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
6.5.1.3 | more |
the group I intron from cyanobacterium Anabaena sp. catalyzes phosphodiester bond formation using a triphosphate on the 5'-terminal nucleotide, much like protein polymerases and engineered ribozymes. In the process, this ribozyme forms a unique circular RNA that incorporates the exogenous guanosine cofactor added during self-splicing |
Anabaena sp. |
? |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
- |
Branchiostoma floridae |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | more |
the RNA ligase has a a strict requirement for RNA substrates with a 2'-phosphate terminus for the ligation of RNAs with 5'-phosphate and 3'-hydroxyl ends. RNA ligase forms a 2'-phosphomonoester-3',5'-phosphodiester junction |
Branchiostoma floridae |
? |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
DraRnl seals 3'-OH/5'-PO4 RNA nicks in either a duplex RNA or an RNA:DNA hybrid, but it cannot seal 3'-OH/5'-PO4 DNA nicks |
Deinococcus radiodurans |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m |
- |
Diplonema papillatum |
(ribonucleotide)n+m + AMP + diphosphate |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
the L1 ligase is regioselective for formation of the biologically relevant 5' to 3' phosphodiester bond rather than a 5' to 2' bond |
Enterobacteria phage L1 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
T4 RNA ligase 1 can efficiently adenylate DNA oligos with 5' phosphate and 3' blocking group |
Escherichia coli |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | more |
RtcA is able of cyclizing a 2'-phosphate RNA end in high yield. The rate of 2'-phosphate cyclization by RtcA is five orders of magnitude slower than 3'-phosphate cyclization, notwithstanding that RtcA binds with similar affinity to RNA3'p and RNA2'p substrates |
Escherichia coli |
? |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
- |
Homo sapiens |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | dATP + (ribonucleotide)n + (ribonucleotide)m |
- |
Homo sapiens |
dAMP + phosphate + (ribonucleotide)n+m |
- |
? |