EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
5.4.99.64 | isovaleryl-CoA |
wild-type enzyme as well as I90V (HcmA) and I90A (HcmA) mutant enzymes can also isomerize pivalyl- and isovaleryl-CoA, albeit at more than 10times lower rates than the favorite substrate (S)-3-hydroxybutyryl-CoA |
Aquincola tertiaricarbonis |
? |
- |
? |
5.4.99.64 | more |
the enzyme does not show detectable conversion of methylmalonyl-CoA and succinyl-CoA |
Aquincola tertiaricarbonis |
? |
- |
? |
5.4.99.64 | more |
(R)-3-hydroxybuytryl-CoA is converted 3.1 times faster than the (S)-enantiomer |
Kyrpidia tusciae DSM 2912 |
? |
- |
? |
5.4.99.64 | more |
the enzyme does not show detectable conversion of methylmalonyl-CoA and succinyl-CoA |
Aquincola tertiaricarbonis L108 |
? |
- |
? |
5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
poor substrate |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
2-hydroxy-2-methylpropanoyl i.e. 2-hydroxyisobutanoyl-CoA. 2-Hydroxyisobutanoyl-CoA is predominantly converted to (S)-3-hydroxybutanoyl-CoA, and only minor amounts of about 20% of the (R)-enantiomer are obtained |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
very low activity with wild-type enzyme. Mutations D117V or D117A in the large subunit HcmA result in significantly increased activity toward (R)-3-hydroxybutyryl-CoA |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
2-hydroxy-2-methylpropanoyl i.e. 2-hydroxyisobutanoyl-CoA. 2-Hydroxyisobutanoyl-CoA is predominantly converted to (S)-3-hydroxybutanoyl-CoA, and only minor amounts of about 20% of the (R)-enantiomer are obtained |
Aquincola tertiaricarbonis L108 |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
catalytic efficiency for the (R)-enantiomer is about 11 times higher than for the (S)-enantiomer |
Kyrpidia tusciae DSM 2912 |
2-hydroxybutanoyl-CoA |
- |
r |
5.4.99.64 | (S)-3-hydroxybutanoyl-CoA |
- |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |