EC Number   |
Substrates |
Organism |
Products  |
Reversibility |
|---|
   5.4.99.64 | 2-hydroxy-2-methylpropanoyl-CoA |
- |
Aquincola tertiaricarbonis |
(S)-3-hydroxybutanoyl-CoA |
- |
r |
| 5.4.99.64 | 2-hydroxy-2-methylpropanoyl-CoA |
the enzym plays a central role in the degradation of assorted substrates containing a tert-butyl moiety |
Aquincola tertiaricarbonis |
(S)-3-hydroxybutanoyl-CoA |
- |
r |
| 5.4.99.64 | 2-hydroxy-2-methylpropanoyl-CoA |
2-hydroxy-2-methylpropanoyl i.e. 2-hydroxyisobutanoyl-CoA. 2-Hydroxyisobutanoyl-CoA is predominantly converted to (S)-3-hydroxybutanoyl-CoA, and only minor amounts of about 20% of the (R)-enantiomer are obtained, indicating stereospecific catalysis. The enzyme uses radical chemistry to rearrange the positions of both a methyl group and a hydroxyl group. Compared with Vmax obtained with 2-hydroxyisobutanoyl-CoA, rates with (S)-3-hydroxybutanoyl-CoA are nearly four times higher |
Aquincola tertiaricarbonis |
(S)-3-hydroxybutanoyl-CoA |
- |
r |
| 5.4.99.64 | 2-hydroxy-2-methylpropanoyl-CoA |
the enzym plays a central role in the degradation of assorted substrates containing a tert-butyl moiety |
Aquincola tertiaricarbonis L108 |
(S)-3-hydroxybutanoyl-CoA |
- |
r |
| 5.4.99.64 | 2-hydroxy-2-methylpropanoyl-CoA |
2-hydroxy-2-methylpropanoyl i.e. 2-hydroxyisobutanoyl-CoA. 2-Hydroxyisobutanoyl-CoA is predominantly converted to (S)-3-hydroxybutanoyl-CoA, and only minor amounts of about 20% of the (R)-enantiomer are obtained, indicating stereospecific catalysis. The enzyme uses radical chemistry to rearrange the positions of both a methyl group and a hydroxyl group. Compared with Vmax obtained with 2-hydroxyisobutanoyl-CoA, rates with (S)-3-hydroxybutanoyl-CoA are nearly four times higher |
Aquincola tertiaricarbonis L108 |
(S)-3-hydroxybutanoyl-CoA |
- |
r |
| 5.4.99.64 | 2-hydroxyisobutanoyl-CoA |
- |
Kyrpidia tusciae DSM 2912 |
(S)-3-hydroxyisobutanoyl-CoA + (R)-3-hydroxyisobutanoyl-CoA |
- |
r |
| 5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
poor substrate |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
| 5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
2-hydroxy-2-methylpropanoyl i.e. 2-hydroxyisobutanoyl-CoA. 2-Hydroxyisobutanoyl-CoA is predominantly converted to (S)-3-hydroxybutanoyl-CoA, and only minor amounts of about 20% of the (R)-enantiomer are obtained |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
| 5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
very low activity with wild-type enzyme. Mutations D117V or D117A in the large subunit HcmA result in significantly increased activity toward (R)-3-hydroxybutyryl-CoA |
Aquincola tertiaricarbonis |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
| 5.4.99.64 | (R)-3-hydroxybutanoyl-CoA |
2-hydroxy-2-methylpropanoyl i.e. 2-hydroxyisobutanoyl-CoA. 2-Hydroxyisobutanoyl-CoA is predominantly converted to (S)-3-hydroxybutanoyl-CoA, and only minor amounts of about 20% of the (R)-enantiomer are obtained |
Aquincola tertiaricarbonis L108 |
2-hydroxy-2-methylpropanoyl-CoA |
- |
r |
