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Results 1 - 6 of 6
EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene
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cucurbitadienol
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?
(3S)-2,3-epoxy-2,3-dihydrosqualene
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cucurbitadienol
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?
(3S)-2,3-epoxy-2,3-dihydrosqualene
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cucurbitadienol
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?
(S)-squalene-2,3-epoxide
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cucurbitadienol
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?
more
GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol
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more
11-oxo-24,25-epoxy cucurbitadienol might be a derivative of 24,25-epoxy cucurbitadienol, which is assumed to be formed through cyclization of 2,3-(S)-22,23-(S)-dioxidosqualene in yeast cells by recombinant SgCbQ, and then sequentially oxidized by recombinant CYP87D18 (EC 1.14.14.71) at the C-11 position in BY4741-Z5 as the oxidation process of cucurbitadienol. Metabolite identification by TLC, metabolite structures are confirmed by liquid chromatography-solid-phase extraction-nuclear magnetic resonance-mass spectrometry coupling (LC-SPE-NMR-MS)
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Results 1 - 6 of 6