EC Number   |
Substrates  |
Organism |
Products |
Reversibility |
|---|
    4.4.1.22 | 5,6,7,8-tetrahydromethanopterin + formaldehyde |
- |
Paracoccus denitrificans |
5,10-methylenetetrahydromethanopterin + H2O |
- |
? |
| 4.4.1.22 | glutathione + formaldehyde |
- |
Paracoccus denitrificans |
S-(hydroxymethyl)glutathione |
- |
? |
| 4.4.1.22 | more |
the enzyme glutathione-dependent formaldehyde-activating enzyme (GFA) from Paracoccus denitrificans catalyzes S-hydroxymethylglutathione (HMG) formation from glutathione and formaldehyde. Enzyme GFA binds glutathione but does not directly catalyse HMG formation under standard conditions. It is possible that GFA acts as a glutathione carrier that acts to co-localise glutathione and formaldehyde in a cellular context. NMR exchange spectroscopy is used for ligand identification. Glutathione binding structure, overview |
Paracoccus denitrificans |
? |
- |
? |
| 4.4.1.22 | S-(hydroxymethyl)glutathione |
- |
Paracoccus denitrificans |
glutathione + formaldehyde |
- |
? |
| 4.4.1.22 | S-(hydroxymethyl)glutathione |
the enzyme accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway |
Paracoccus denitrificans |
glutathione + formaldehyde |
- |
? |
