EC Number   |
Substrates |
Organism |
Products |
Reversibility  |
|---|
   3.5.5.5 | more |
activity of less than 1% compared to the activity with madelonitrile: fumaronitrile, 3-hydroxyglutaronitrile, succinonitrile, 3-hydroxypropionitrile. The enzyme hydrolyzes nitriles with diverse structures. Arylacetonitriles are the optimal substrates |
Pseudomonas psychrotolerans |
? |
- |
- |
| 3.5.5.5 | more |
the enzyme shows preference for unsaturated aliphatic substrates containing 5-6 carbon atoms. Increased reaction rates are also found for aliphatic nitriles carrying electron withdrawing substituents (e.g. chloro- or hydroxy-groups) close to the nitrile group. Aliphatic dinitriles are attacked only at one of the nitrile groups and with most of the tested dinitriles the monocarboxylates are detected as major products. Substrates converted with 1% less compared to the activity with 3-hexenedinitrile: 2-methylbutyronitrile, decanedinitrile, octanedinitrile, cis,trans-crotononitrile, popionitrile, isovaleronitrile, lactonitrile, butyronitrile, |
Pseudomonas fluorescens |
? |
- |
- |
| 3.5.5.5 | more |
very low activity with: (R,S)-Mandelonitrile |
Trichoderma virens Gv29-8 |
? |
- |
- |
| 3.5.5.5 | more |
very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine |
Auricularia delicata |
? |
- |
- |
| 3.5.5.5 | more |
very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine |
Aspergillus luchuensis |
? |
- |
- |
| 3.5.5.5 | more |
very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine |
Macrophomina phaseolina |
? |
- |
- |
| 3.5.5.5 | more |
the enzyme shows preference for unsaturated aliphatic substrates containing 5-6 carbon atoms. Increased reaction rates are also found for aliphatic nitriles carrying electron withdrawing substituents (e.g. chloro- or hydroxy-groups) close to the nitrile group. Aliphatic dinitriles are attacked only at one of the nitrile groups and with most of the tested dinitriles the monocarboxylates are detected as major products. Substrates converted with 1% less compared to the activity with 3-hexenedinitrile: 2-methylbutyronitrile, decanedinitrile, octanedinitrile, cis,trans-crotononitrile, popionitrile, isovaleronitrile, lactonitrile, butyronitrile, |
Pseudomonas fluorescens EBC191 |
? |
- |
- |
| 3.5.5.5 | more |
very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine |
Macrophomina phaseolina MS6 |
? |
- |
- |
| 3.5.5.5 | more |
very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine |
Auricularia delicata TFB-10046 SS5 |
? |
- |
- |
| 3.5.5.5 | more |
very low activity with: benzonitrile, 2-cyanopyridine, 3-cyanopyridine, 4-cyanopyridine |
Aspergillus luchuensis IFO 4308 |
? |
- |
- |
