EC Number   |
Substrates |
Products |
Reversibility |
|---|
   3.5.1.119 | more |
the enzyme is unable to hydrolyze linear Pup-substrate fusions |
? |
- |
? |
| 3.5.1.119 | more |
enzyme acts as a depupylase in the Pup proteasome system in vivo and in vitro. Dop removes prokaryotic ubiquitin-like protein Pup from substrates by specific cleavage of the isopeptide bond |
? |
- |
? |
| 3.5.1.119 | more |
steric hindrance in positioning the isopeptide bond in the active site of Dop might be responsible for the observed differences in depupylation rather than differences in the overall affinity of pupylated substrate for Dop |
? |
- |
? |
| 3.5.1.119 | N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[His6-myo-inositol-1-phosphate synthetase]-L-lysine + H2O |
- |
[prokaryotic ubiquitin-like protein]-L-glutamate + [His6-myo-inositol-1-phosphate synthetase]-L-lysine |
- |
? |
| 3.5.1.119 | N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine + H2O |
- |
[prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine |
- |
? |
| 3.5.1.119 | N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine + H2O |
the enzyme catalyzes the nucleophilic attack of water on the terminal carbonyl atom of Pup, releasing ammonium in the case of deamidation or the substrate lysine in case of depupylation |
[prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine |
- |
? |
| 3.5.1.119 | [prokaryotic ubiquitin-like protein]-fluorescein-5-carboxamide-L-lysine + H2O |
hydrolysis of the isopeptide bond in Pup-Fl and release the fluorescein-5-carboxamide lysine moiety |
[prokaryotic ubiquitin-like protein]-L-lysine + 5-carboxyfluorescein |
- |
? |
| 3.5.1.119 | [prokaryotic ubiquitin-like protein]-gamma-L-glutamyl-[Bcp protein]-L-lysine + H2O |
- |
[prokaryotic ubiquitin-like protein]-L-glutamate + [Bcp protein]-L-lysine |
- |
? |
| 3.5.1.119 | [prokaryotic ubiquitin-like protein]-gamma-L-glutamyl-[FabD protein]-L-lysine + H2O |
- |
[prokaryotic ubiquitin-like protein]-L-glutamate + [FabD protein]-L-lysine |
- |
? |
| 3.5.1.119 | [prokaryotic ubiquitin-like protein]-gamma-L-glutamyl-[FadA protein]-L-lysine + H2O |
- |
[prokaryotic ubiquitin-like protein]-L-glutamate + [FadA protein]-L-lysine |
- |
? |
