EC Number   |
Substrates  |
Organism |
Products |
Reversibility |
|---|
   3.4.22.B74 | (SUMO-1)-beta-galactosidase conjugate + H2O |
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3-beta-galactosidase |
Homo sapiens |
SUMO-1 + beta-galactosidase |
- |
? |
| 3.4.22.B74 | (SUMO-3)-Ran GTPase-activating protein 1 conjugate + H2O |
SENP6 and SENP7 prefer SUMO2 or SUMO3 in deconjugation reactions with rates comparable with those catalyzed by SENP2. In contrast to SENP2, SENP6 and SENP7 are less able to deconjugate SUMO1-RanGAP1, and products are only detected at enzyme concentrations 100 x higher than that observed for reactions containing SENP2 |
Homo sapiens |
SUMO-3 + Ran GTPase-activating protein 1 |
- |
? |
| 3.4.22.B74 | acetyl-LRGG-7-amido-4-trifluoromethylcoumarin + H2O |
SENP6 prefers the LRGG sequence, residues typical of Nedd8 or ubiquitin in the P3 and P4 positions |
Homo sapiens |
acetyl-LRGG + 7-amino-4-trifluoromethylcoumarin |
- |
? |
| 3.4.22.B74 | acetyl-QTGG-7-amido-4-trifluoromethylcoumarin + H2O |
very low activity |
Homo sapiens |
acetyl-QTGG + 7-amino-4-trifluoromethylcoumarin |
- |
? |
| 3.4.22.B74 | di-SUMO-2 + H2O |
- |
Homo sapiens |
2 SUMO-2 |
- |
? |
| 3.4.22.B74 | di-SUMO-3 + H2O |
- |
Homo sapiens |
2 SUMO-3 |
- |
? |
| 3.4.22.B74 | more |
although SUMO-2/3 chains are the preferred substrate, SENP6 is also capable of cleaving mixed chains of SUMO-1 and SUMO-2/3 |
Homo sapiens |
? |
- |
? |
| 3.4.22.B74 | more |
SENP6 reveals no detectable endopeptidase activity on the precursors of ubiquitin, Nedd8 and ISG15 |
Homo sapiens |
? |
- |
? |
| 3.4.22.B74 | more |
SUSP1 expressed in Escherichia coli cells efficiently releases SUMO-1 from SUMO-1-beta-galactosidase fusion but not from other ubiquitin-like protein fusions, including Smt3zb-galactosidase, suggesting its role in the generation of matured SUMO-1 specifically from its precursors |
Homo sapiens |
? |
- |
? |
| 3.4.22.B74 | more |
isozyme SENP6 shows isoform specificity for SUMO2/3 over SUMO1 |
Homo sapiens |
? |
- |
? |
