EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.1.1.84 | (-)-cocaine + H2O |
- |
Rhodococcus sp. |
ecgonine methyl ester + benzoate |
- |
? |
| 3.1.1.84 | (-)-cocaine + H2O |
- |
Homo sapiens |
ecgonine methyl ester + benzoate |
- |
? |
| 3.1.1.84 | (-)-cocaine + H2O |
(-)-cocaine is the naturally occurring enantiomer. The entire hydrolysis reaction consists of four reaction steps, including the nucleophilic attack on the carbonyl carbon of benzoyl ester group by the hydroxyl group of Ser117, dissociation of benzoyl ester group, nucleophilic attack on the carbonyl carbon of benzoyl ester group by water, and finally dissociation between the (-)-cocaine benzoyl group and Ser117 of CocE. The third reaction step involving the nucleophilic attack of a water molecule is rate-determining |
Rhodococcus sp. |
ecgonine methyl ester + benzoate |
- |
? |
| 3.1.1.84 | 4-methylumbelliferyl acetate + H2O |
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 |
Homo sapiens |
4-methylumbelliferone + acetate |
- |
? |
| 3.1.1.84 | 6-monoacetylmorphine + H2O |
- |
Homo sapiens |
morphine + acetate |
- |
? |
| 3.1.1.84 | 6-monoacetylmorphine + H2O |
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 |
Homo sapiens |
morphine + acetate |
- |
? |
| 3.1.1.84 | cocaethylene + H2O |
cocaethylene is a more potent cocaine metabolite, observed in patients who concurrently abuse cocaine and alcohol |
Rhodococcus sp. |
? |
- |
? |
| 3.1.1.84 | cocaine + H2O |
- |
Rhodococcus sp. |
ecgonine methyl ester + benzoate |
- |
? |
| 3.1.1.84 | cocaine + H2O |
- |
Stenotrophomonas maltophilia |
ecgonine methyl ester + benzoate |
- |
? |
| 3.1.1.84 | cocaine + H2O |
CocE is the most efficient native enzyme for metabolizing the naturally occurring cocaine |
Rhodococcus sp. |
ecgonine methyl ester + benzoate |
- |
? |
