EC Number   |
Substrates |
Organism |
Products |
Reversibility  |
|---|
  2.7.7.B5 | ATP + TPHE39A |
truncated tRNA consisting of 39 nucleotides derived from tRNAPhe, minimal RNA substrate for modification by ThiI. The crystal structure of TPHE39A shows that base pairs in the T-stem are almost completely disrupted, although those in the acceptor-stem are preserved. ThiI can efficiently bind with not only tRNAPhe but also TPHE39A |
Escherichia coli |
adenylated TPHE39A + diphosphate |
- |
? |
| 2.7.7.B5 | more |
ThiI is involved in the modification of uridine to 4-thiouridine |
Escherichia coli |
? |
- |
? |
| 2.7.7.B5 | more |
ThiI plays a role in the transfer of sulfur from cysteine to both thiamine and 4-thiouridine |
Escherichia coli |
? |
- |
? |
| 2.7.7.B5 | tRNA-uridine + ATP |
- |
Escherichia coli |
adenylated-trNA-uridine + diphosphate |
- |
? |
| 2.7.7.B5 | tRNA-uridine + ATP |
based upon sequence alignments, ThiI shares a unique P-loop motif with the PPi synthetase family. ThiI very likely catalyzes the adenylation of a substrate, probably a molecule bearing a carbonyl oxygen |
Escherichia coli |
adenylated-trNA-uridine + diphosphate |
- |
? |
| 2.7.7.B5 | tRNA-uridine + GTP |
kcat/Km for GTP is about 6fold lower than the value for ATP |
Escherichia coli |
adenylated-trNA-uridine + diphosphate |
- |
? |
