EC Number |
Substrates |
Products |
Reversibility |
---|
2.4.1.B34 | amylopectin + acceptor |
transglycosylation activity |
? |
- |
? |
2.4.1.B34 | amylopectin + H2O |
hydrolytic activity |
? |
- |
? |
2.4.1.B34 | amylose + maltose |
the enzyme synthesizes larger saccharides with alpha1->4 and alpha1->6 glucosidic linkages |
maltotriose + panose |
panose i.e. Glc-alpha-(1->6)-maltose |
? |
2.4.1.B34 | amylose V |
- |
? |
- |
? |
2.4.1.B34 | amylose V |
- |
? |
GtfB produces a high molecular mass polymer with a molecular mass about 80 times greater than that of the starting amylose V |
? |
2.4.1.B34 | amylose V + acceptor |
transglycosylation activity |
? |
- |
? |
2.4.1.B34 | amylose V + H2O |
- |
? |
both hydrolysis and transferase activities occur. Besides the presence of alpha1->4 linkages, alpha1->6 linkages are newly formed. In the product mixture derived from amylose V, the alpha1->6/alpha1->4 linkage ratio increases up to 90:10. In the product mixture, free glucose, 4-substituted reducing glucose residues [-(1->4)-alpha-D-Glc], and a small amount of reducing-end glucose residues which are 6 substituted are detected |
? |
2.4.1.B34 | amylose V + H2O |
hydrolytic activity |
? |
- |
? |
2.4.1.B34 | amylose V + H2O |
hydrolytic activity, amylose V with Mw 170 kDa |
? |
- |
? |
2.4.1.B34 | amylose V + maltoheptaose |
transglycosylation activity |
? |
- |
? |