EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.3.1.193 | [elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA |
tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop |
Escherichia coli |
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A |
- |
? |
2.3.1.193 | [elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA |
ATP/GTP hydrolysis by TmcA is stimulated in the presence of acetyl-CoA and tRNAMet. TmcA hydrolyses ATP or GTP between the beta- and gamma-phosphates, as shown by the detection of labelled ADP (or GDP). A mutation study reveals that Escherichia coli TmcA strictly discriminates elongator tRNAMet from the structurally similar tRNAIle by mainly recognizing the C27G43 pair in the anticodon stem |
Escherichia coli |
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A |
- |
? |
2.3.1.193 | [elongator tRNAMet]-cytidine34 + GTP + acetyl-CoA |
- |
Escherichia coli |
[elongator tRNAMet]-N4-acetylcytidine34 + GDP + phosphate + coenzyme A |
- |
? |