EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.300 | more |
analysis of substrate specificity by mass spectrometry, overview. Enzyme PavNMT exhibits a preference for (+)-pavine and the 1-benzylisoquinoline (S)-reticuline, but also accepts the protoberberines scoulerine and stylopine and, to a lesser extent, tetrahydropapaverine (THP). No activity with (R)-reticuline and papaverine, as well as cryptopine, glaucine, codeine, canadaline, noscapine, and berbamine. Structural determinants of substrate binding are derived from crystal structure analysis |
Thalictrum flavum subsp. glaucum |
? |
- |
- |
2.1.1.300 | S-adenosyl-L-methionine + (+/-)-N-methylpavine |
- |
Thalictrum flavum subsp. glaucum |
S-adenosyl-L-homocysteine + (+/-)-N,N-dimethylpavine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (+/-)-pavine |
- |
Thalictrum flavum |
S-adenosyl-L-homocysteine + N-methylpavine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (+/-)-pavine |
- |
Thalictrum flavum subsp. glaucum |
S-adenosyl-L-homocysteine + (+/-)-N-methylpavine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (R,S)-scoulerine |
- |
Thalictrum flavum subsp. glaucum |
S-adenosyl-L-homocysteine + N-methylscoulerine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (R,S)-stylopine |
- |
Thalictrum flavum |
S-adenosyl-L-homocysteine + N-methylstylopine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (R,S)-stylopine |
- |
Thalictrum flavum subsp. glaucum |
S-adenosyl-L-homocysteine + N-methylstylopine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (S)-reticuline |
best substrate. (R)-reticuline is not accepted as a substrate, revealing a strong stereoselectivity for (S)-reticuline |
Thalictrum flavum subsp. glaucum |
S-adenosyl-L-homocysteine + (S)-tetrahydropapaverine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (S)-scoulerine |
- |
Thalictrum flavum |
S-adenosyl-L-homocysteine + N-methyl-(S)-scoulerine |
- |
? |
2.1.1.300 | S-adenosyl-L-methionine + (S)-tetrahydropapaverine |
- |
Thalictrum flavum subsp. glaucum |
S-adenosyl-L-homocysteine + (S)-laudanosine |
- |
- |