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EC Number Substrates Commentary Substrates Products Commentary (Products) Reversibility
Display the reaction diagram Show all sequences 2.1.1.283more the enzyme shows a broad substrate specificity. SpOMT7740 catalyzes the O-methylation reaction to form various natural and non-natural O-methoxides, includes 7-hydroxy-8-O-methoxy flavone, 3-O-methoxy flavone, three mono-, di-, and tri-O-methoxy genistein, mono-O-methoxy phloretin, mono-O-methoxy luteolin, 3-O-methoxy beta-sitosterol, and O-methoxy anthraquinones (emodin and aloe emodin) and O-methoxy anthracycline (daunorubicin) exhibiting diverse substrate flexibility. Mass spectrometric analysis of reaction products. 7,8-Dihydroxyflavone is the best substrate for bioconversion to 7-hydroxy-8-O-methoxy flavone. The enzyme seems to prefer methylation of hydroxy groups at C8 position. No or poor activity with doxorubicin and daunorubicin ? - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone i.e. citreorosein, 22% of the activity compared to emodin ? - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone - S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + 3-hydroxyflavone lower activity S-adenosyl-L-homocysteine + 3-methoxyflavone - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + 7,8-dihydroxyflavone best substrate S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + 7-chloroemodin 18% of the activity compared to emodin ? - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + aloe emodin low activity S-adenosyl-L-homocysteine + 8-methoxy-1-hydroxy-3-(hydroxymethyl)anthracene-9,10-dione - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + beta-sitosterol low activity S-adenosyl-L-homocysteine + 3-methoxy-beta-sitosterol - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + catenarin 80% of the activity compared to emodin ? - ?
Display the reaction diagram Show all sequences 2.1.1.283S-adenosyl-L-methionine + emodin the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin S-adenosyl-L-homocysteine + questin - ?
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