EC Number   |
Substrates |
Organism |
Products |
Reversibility  |
|---|
   2.1.1.202 | more |
C34 methylation depends on Trm4a in both tRNALeuCAA and tRNAProCGG. Substrate specificity, comparison to trm4b, overview |
Schizosaccharomyces pombe |
? |
- |
- |
| 2.1.1.202 | more |
susbtrate specificity of NSUN2, overview. NSUN2 has a much broader target spectrum and is able to modify several positions (C34, C40, C48, C49, and C50) in a number of different tRNAs, as well as other RNA substrates. The enzyme is also active with various mRNAs. M5C modifications in cytoplasmic and mitochondrial tRNAs. Three-dimensional L-shape structure of a tRNA with the positions of m5C modifications and the cognate methyltransferases responsible for installing them marked. The m5C modifications in cytoplasmic and mitochondrial tRNAs. Schematic secondary structure and three-dimensional L-shape structure of a tRNA with the positions of m5C modifications. The broad-spectrum methyltransferase NSUN2 has been suggested to recognize different features in its diverse substrate RNAs. The reported NSUN2-mediated m5C modifications in mRNAs typically lie within highly GC-rich regions, suggesting that the enzyme may preferentially bind such sequences. But all the known NSUN2-mediated m5C modifications in vtRNAs lie within a UCG motif, and mutagenic analysis of the NSUN2 target pre-tRNALeu reveals a consensus sequence of C/A/U32-U/A33-m5C34-A35-A36-G37 |
Homo sapiens |
? |
- |
- |
| 2.1.1.202 | more |
susbtrate specificity of NSUN6, overview |
Pyrococcus horikoshii |
? |
- |
- |
| 2.1.1.202 | more |
susbtrate specificity of NSUN6, overview. Three-dimensional L-shape structure of a tRNA with the positions of m5C modifications and the cognate methyltransferases responsible for installing them marked. The m5C modifications in cytoplasmic and mitochondrial tRNAs. Schematic secondary structure and three-dimensional L-shape structure of a tRNA with the positions of m5C modifications, determination of the interaction sites of NSUN6 with the discriminator base and additional base pairs in the acceptor stem and the D-loop, as observed by X-ray crystallography, overview. m5C72 modifications installed by NSUN6 lie within the acceptor stem of tRNA. NSUN6 forms extensive contacts with its substrate tRNAs. Binding of NSUN6 disrupts base pairing within the tRNA acceptor stem and promotes base-flipping of C71 to make the C5 atom of the C72 nucleotide, which is normally base paired with G1, accessible for methylation. NSUN6 also has a PUA domain that binds to the D-stem region of substrate tRNAs, as well as the non-genomically encoded CCA 3' end. Consistent with this binding mode, the presence of the CCA is found to be an essential pre-requisite for methylation of tRNACys and tRNAThr by NUSN6 |
Homo sapiens |
? |
- |
- |
| 2.1.1.202 | more |
the CCA end is precisely recognized by hNSun6 primarily through the PUA domain, overview. The main chains of the residues (Arg126, Pro206 and Asp209) recognizes C74. Recognition for C75 comes from the main chain residues (Lys192 and Gly193) and the side chain residues (Lys192 and Asp209). The base moiety of C75 is stacked with the Tyr131 residue. Recognition for A76 is achieved mostly by the main chain of His129 and the side chain of Lys 192. Hydrophobic interactions with ambient aa residues, including Cys120, facilitate localization of the A76 base moiety. The discriminator base U73 binds to the RRM motif. C72 is recognized by the catalytic core. Multiple roles of the PUA domain in tRNA binding |
Homo sapiens |
? |
- |
- |
| 2.1.1.202 | more |
Trm4b is active on the wild-type tRNA, its activity is slightly enhanced on C34A and completely abrogated on C49A, indicating that Trm4b has in vitro activity on tRNAProCGG and is specific for C49, which is in agreement with in vivo methylation data. Substrate specificity, comparison to trm4a, overview |
Schizosaccharomyces pombe |
? |
- |
- |
| 2.1.1.202 | more |
susbtrate specificity of NSUN6, overview |
Pyrococcus horikoshii DSM 12428 |
? |
- |
- |
| 2.1.1.202 | more |
Trm4b is active on the wild-type tRNA, its activity is slightly enhanced on C34A and completely abrogated on C49A, indicating that Trm4b has in vitro activity on tRNAProCGG and is specific for C49, which is in agreement with in vivo methylation data. Substrate specificity, comparison to trm4a, overview |
Schizosaccharomyces pombe ATCC 24843 |
? |
- |
- |
| 2.1.1.202 | more |
C34 methylation depends on Trm4a in both tRNALeuCAA and tRNAProCGG. Substrate specificity, comparison to trm4b, overview |
Schizosaccharomyces pombe ATCC 24843 |
? |
- |
- |
| 2.1.1.202 | more |
susbtrate specificity of NSUN6, overview |
Pyrococcus horikoshii NBRC 100139 |
? |
- |
- |
