EC Number   |
Substrates |
Products |
Reversibility |
|---|
   2.1.1.189 | more |
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation |
? |
- |
? |
| 2.1.1.189 | more |
substrate specificity, overview |
? |
- |
? |
| 2.1.1.189 | more |
the enzyme also methylates uracil1939 in 23S rRNA |
? |
- |
? |
| 2.1.1.189 | more |
enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 |
? |
- |
? |
| 2.1.1.189 | more |
enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 |
? |
- |
? |
| 2.1.1.189 | more |
replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation |
? |
- |
? |
| 2.1.1.189 | more |
in the archaeon Pyrococcus horikoshii, the m5U methylation sites in domains II and IV of 23S rRNA are located at U875 (U747 in Escherichia coli) and U2055 (U1939 in Escherichia coli) of the hairpin 35, cf. EC 2.1.1.190 |
? |
- |
- |
| 2.1.1.189 | S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3' |
18-mer RNA analogue of the 23S rRNA helix 35 |
S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3' |
- |
? |
| 2.1.1.189 | S-adenosyl-L-methionine + uracil747 in 23S rRNA |
it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain |
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA |
- |
? |
| 2.1.1.189 | S-adenosyl-L-methionine + uracil747 in 23S rRNA |
- |
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA |
- |
? |
