EC Number   |
Substrates |
Organism |
Products  |
Reversibility |
|---|
    1.4.1.13 | more |
- |
Escherichia coli |
? |
- |
? |
| 1.4.1.13 | more |
the enzyme beta subunit is devoid of glutamate synthase activity in either direction at both pH 7.5 and 9.5, but it can oxidize NADPH and transfer electrons to synthetic electron acceptors like iodonitrotetrazolium, ferricyanide, menadione, dichloroindophenol, the beta subunit is highly specific toward NADPH, the rate of oxidation of NADH in the presence of electron acceptors is less than 5% of that measured with NADPH |
Azospirillum brasilense |
? |
- |
? |
| 1.4.1.13 | more |
NH4Cl, L-asparagine, D-glutamine, or alkylated glutamine analogues do not substitute for L-glutamine, pyruvate or oxalacetate do not substitute for alpha-ketoglutarate |
Escherichia coli |
? |
- |
? |
| 1.4.1.13 | more |
the recombinant enzyme has diaphorase activity, it can oxidize NADPH and transfer electrons to synthetic electron acceptors like iodonitrotetrazolium and ferricyanide |
Azospirillum brasilense |
? |
- |
? |
| 1.4.1.13 | more |
NH3-dependent activity is increased approximately 5fold in apoglutamate synthase lacking flavin and non-heme iron |
Escherichia coli |
? |
- |
? |
| 1.4.1.13 | more |
glutamine binding site of the enzyme is located on the heavy subunit of the enzyme, preparations of the enzyme that lack flavins or the flavins and iron sulfide catalyze NH3-dependent reaction but not glutamine-dependent reaction |
Klebsiella aerogenes |
? |
- |
? |
| 1.4.1.13 | more |
amino acids, amines and ammonium chloride do not substitute for L-glutamine, other alpha-keto acids including oxalacetate, pyruvate, glyoxylate and alpha-ketobutyrate do not support the activity |
Gluconobacter oxydans |
? |
- |
? |
| 1.4.1.13 | more |
alpha-ketoglutarate can not be replaced with pyruvate or oxalacetate |
Thiobacillus thioparus |
? |
- |
? |
| 1.4.1.13 | more |
under conditions of physiological pH the enzyme exhibits a reversible half-reaction, but overall catalysis is essentially irreversible |
Azospirillum brasilense |
? |
- |
? |
| 1.4.1.13 | more |
the alpha subunit catalyzes the synthesis of glutamate from L-glutamine and 2-oxoglutarate, provided that a reducing system is present, reducing system: dithionite and methyl viologen |
Azospirillum brasilense |
? |
- |
? |
