EC Number   |
Substrates |
Products |
Reversibility |
|---|
   1.14.99.53 | colloidal chitin + ascorbate + O2 |
- |
oxidized chitin oligosaccharides + dehydroascorbate + H2O |
with colloidal chitin as the substrate, a ladder of oxidized oligosaccharides is observed |
? |
| 1.14.99.53 | chitin + acceptor + O2 |
- |
[chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone |
the products are a series of chitin oligosaccharides in aldonic acid or lactone form with varying degree of polymerization: DPox4 > DPox5 > DPox6 > DPox7 > DPox8. Oligosaccharides with even-numbered chain lengths are predominant. |
? |
| 1.14.99.53 | beta-chitin + acceptor + O2 |
beta chitin from squid pen, best substrate |
oligosaccharide aldonic acids + reduced acceptor + H2O |
strong preference towards even-numbered products |
? |
| 1.14.99.53 | alpha-chitin + ascorbate + O2 |
enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin |
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O |
products show oxidation of the C1 carbon leading to aldonic acids |
? |
| 1.14.99.53 | beta-chitin + ascorbate + O2 |
enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin |
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O |
products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids |
? |
| 1.14.99.53 | alpha-chitin + ascorbate + O2 |
- |
chito-oligosaccharide aldonic acid + dehydroascorbate + H2O |
products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation |
? |
| 1.14.99.53 | beta-chitin + acceptor + O2 |
- |
chito-oligosaccharide aldonic acid + reduced acceptor + H2O |
products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation |
? |
| 1.14.99.53 | alpha-chitin + ascorbate + O2 |
- |
? + dehydroascorbate + H2O |
products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom |
? |
| 1.14.99.53 | beta-chitin + ascorbate + O2 |
- |
? + dehydroascorbate + H2O |
products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom |
? |
| 1.14.99.53 | alpha-chitin + ascorbate + O2 |
- |
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O |
products are C1-oxidized chitooligomers, mainly tetramers and hexamers |
? |
