EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.15.8 | 4-androstene-3,17-dione + reduced ferredoxin + O2 |
Kd value of androstenedione is 0.387 mM |
Bacillus sp. PAMC 23377 |
15beta-hydroxyandrost-4-en-3,17-dione + oxidized ferredoxin + H2O |
- |
? |
1.14.15.8 | nandrolone + reduced ferredoxin + O2 |
- |
Bacillus sp. PAMC 23377 |
? + oxidized ferredoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycorticosterone + reduced adrenodoxin + O2 |
- |
Priestia megaterium |
15beta-hydroxy-11-deoxycorticosterone + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycorticosterone + reduced adrenodoxin + O2 |
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland, 15beta-hydroxy-deoxycorticosterone is the only product that is observed, both in the case of wild-type CYP106A2 and all the mutants (except T396R) |
Priestia megaterium |
15beta-hydroxy-11-deoxycorticosterone + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycorticosterone + reduced adrenodoxin + O2 |
deoxycorticosterone binds in the heme pocket near the iron ligand |
Priestia megaterium |
15beta-hydroxy-11-deoxycorticosterone + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycorticosterone + reduced adrenodoxin + O2 |
- |
Priestia megaterium |
15beta-hydroxy-11-deoxycorticosterone + 7beta,15beta-dihydroxy-11-deoxycorticosterone + oxidized adrenodoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycorticosterone + reduced ferredoxin + O2 |
ferredoxin from the cyanobacterium Anabaena PCC 7119 |
Priestia megaterium |
15beta-hydroxy-11-deoxycorticosterone + oxidized ferredoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycorticosterone + reduced flavodoxin + O2 |
flavodoxin from the cyanobacterium Anabaena PCC 7119 |
Priestia megaterium |
15beta-hydroxy-11-deoxycorticosterone + oxidized flavodoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycorticosterone + reduced megaredoxin + O2 |
megaredoxin is an iron-sulfur protein of Bacillus megaterium, megaredoxin has an apparent sulfur to iron ratio of 0.98 |
Priestia megaterium |
15beta-hydroxy-11-deoxycorticosterone + oxidized megaredoxin + H2O |
- |
? |
1.14.15.8 | 11-deoxycortisol + reduced adrenodoxin + O2 |
adrenodoxin is a [2Fe-2S] ferredoxin involved in electron transfer from NADPH+ (via a reductase) to cytochrome P-450 in the adrenal gland |
Priestia megaterium |
15beta-15,17,21-trihydroxypregn-4-ene-3,20-dione + oxidized adrenodoxin + H2O |
- |
? |