EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.1.1.267 | more |
product identification by mass spectrometry (HPLC-MS) |
Populus trichocarpa |
? |
- |
- |
1.1.1.267 | more |
VvDxr residues S186, H209, S222, N227 and K228 may participate in binding of the DXP substrate's phosphate moiety. These residues are conserved. H209 is a part of a flexible loop (209-HPXWXMG-115) that closes on the active site upon substrate binding. While H209 interacts with the phosphate group, W211 and M213 shield the remaining part of Dxp from solvent |
Vibrio vulnificus |
? |
- |
- |
1.1.1.267 | more |
VvDxr residues S186, H209, S222, N227 and K228 may participate in binding of the DXP substrate's phosphate moiety. These residues are conserved. H209 is a part of a flexible loop (209-HPXWXMG-115) that closes on the active site upon substrate binding. While H209 interacts with the phosphate group, W211 and M213 shield the remaining part of Dxp from solvent |
Vibrio vulnificus CMCP6 |
? |
- |
- |
1.1.1.267 | (3R,4S)-dihydroxy-5-oxohexylphosphonic acid + NADPH |
- |
Synechocystis sp. |
? + NADP+ |
- |
? |
1.1.1.267 | 1,2-dideoxy-D-threo-3-hexulose 6-phosphate + NADPH + H+ |
i.e. 1-methyl-1-deoxy-D-xylulose 5-phosphate or M-DXP, a substrate analogue whichis inactive with the wild-type but active with mutant W204F |
Synechocystis sp. |
2-C-ethyl-D-erythritol 4-phosphate + NADP+ |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ |
- |
Hevea brasiliensis |
2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ |
NADPH is the preferred cofactor |
Plectranthus barbatus |
2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ |
inverse secondary kinetic isotope effects for both 3-[2H] and 4-[2H]-D-1-deoxyxylulose-5-phosphate during DXR-catalyzed isomerization indicates that the enzyme operates through a retro-aldol/aldol mechanism |
Escherichia coli |
2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH |
- |
synthetic construct |
? + NADP+ |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ |
- |
Escherichia coli |
2-C-methyl-D-erythritol 4-phosphate + NADP+ |
- |
? |