EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ |
the forward reaction is preferred |
Arabidopsis thaliana |
2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ |
- |
r |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ |
- |
Arabidopsis thaliana |
2-C-methyl-D-erythritol 4-phosphate + NADP+ |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ |
second committed step in isopentenyl diphosphate biosynthesis via the plastidial nonmevalonate pathway of isoprenoid biosynthesis, overview |
Arabidopsis thaliana |
2-C-methyl-D-erythritol 4-phosphate + NADP+ |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ |
second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate |
Arabidopsis thaliana |
2-C-methyl-D-erythritol 4-phosphate + NADP+ |
- |
r |
1.1.1.267 | 2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ |
in reaction mixtures containing 0.150.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle |
Arabidopsis thaliana |
? + NADPH |
- |
r |
1.1.1.267 | 2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ |
reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana |
Arabidopsis thaliana |
? + NADPH |
- |
? |
1.1.1.267 | glycolaldehyde phosphate + hydroxyacetone + NADPH |
reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana |
Arabidopsis thaliana |
? + NADP+ |
- |
? |
1.1.1.267 | more |
participation in the control of the 2-C-methyl-D-erythritol 4-phosphate pathway |
Arabidopsis thaliana |
? |
- |
? |
1.1.1.267 | more |
IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206216) is able to fold into at least three different conformations |
Arabidopsis thaliana |
? |
- |
? |
1.1.1.267 | 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ |
- |
Artemisia annua |
2-C-methyl-D-erythritol 4-phosphate + NADP+ |
- |
? |