EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
neither dATP, GTP, CTP, nor UTP can substitute for ATP. RNA ligation at a 3'-OH/5'-PO4 nick in a double-stranded RNA or an RNA-DNA hybrid |
Tequatrovirus T4 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
the TS2126 RNA ligase catalyzes both inter- and intra-molecular single-stranded DNA ligation to more than 50% completion in not more than a few hours at an elevated temperature, although favoring intramolecular ligation on RNA and single-stranded DNA substrates |
Phage TS2126 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
the L1 ligase is regioselective for formation of the biologically relevant 5' to 3' phosphodiester bond rather than a 5' to 2' bond |
Enterobacteria phage L1 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
Trl1 executes the end-healing and end-sealing steps of tRNA splicing, requires a 2'-PO4 end for tRNA splicing in vivo |
Saccharomyces cerevisiae |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
Rnl1 has an inherent specificity for sealing tRNA with a break in the anticodon loop. The tRNA specificity is imparted by the C domain |
Tequatrovirus T4 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
T4 RNA ligase 1 can efficiently adenylate DNA oligos with 5' phosphate and 3' blocking group |
Escherichia coli |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
the kinetic mechanism of single-turnover nick sealing by T4 Rnl2-AMP is explored by using a rapid mix-quench method and the effects of 3'-OH mispairs and base damage lesions on the rates of nick 5'-adenylylation and phosphodiester synthesis is determined. With respect to the sealing of perfectly paired nicks the rates of step 2 catalysis are rapid (9.5-17.9/sec) and similar in magnitude to the step 3 rates (7.9-32/sec). Rnl2 is kinetically sensitive to all 3'-OH base mispairs |
Tequatrovirus T4 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
- |
Leishmania tarentolae UC |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
possibly involved in posttranscriptional RNA editing process of mitochondrial transcripts |
Leishmania tarentolae |
? |
- |
? |
6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
possibly involved in posttranscriptional RNA editing process of mitochondrial transcripts |
Leishmania tarentolae UC |
? |
- |
? |