EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
   4.2.1.108 | 4-N-acetyl-L-2,4-diaminobutanoate |
final step in L-ectoine biosynthesis, L-ectoine is the compatible solute in the cell |
Halomonas elongata |
L-ectoine + H2O |
- |
? |
| 4.2.1.108 | 4-N-acetyl-L-2,4-diaminobutanoate |
final step in L-ectoine biosynthesis, pathway overview |
Halomonas elongata |
L-ectoine + H2O |
- |
? |
| 4.2.1.108 | 4-N-acetyl-L-2,4-diaminobutanoate |
final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M |
Halomonas elongata |
L-ectoine + H2O |
- |
r |
| 4.2.1.108 | 4-N-acetyl-L-2,4-diaminobutanoate |
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid |
Halomonas elongata |
L-ectoine + H2O |
- |
? |
| 4.2.1.108 | 4-N-acetyl-L-2,4-diaminobutanoate |
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid |
Halomonas elongata |
L-ectoine + H2O |
NMR and thin layer chromatography product identification |
r |
| 4.2.1.108 | 4-N-acetyl-L-2,4-diaminobutanoate |
i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, circularization, L-ectoine as an effective osmoprotectant, the enzyme shows absolute substrate specificity, reversibility of the reaction is not detected but might be below detection range |
Halomonas elongata |
L-ectoine + H2O |
- |
? |
| 4.2.1.108 | D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O |
- |
Halomonas elongata |
? |
- |
r |
| 4.2.1.108 | L-glutamine |
- |
Halomonas elongata |
5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O |
- |
r |
| 4.2.1.108 | L-homoectoine + H2O |
- |
Halomonas elongata |
? |
- |
r |
| 4.2.1.108 | more |
ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview |
Halomonas elongata |
? |
- |
? |
