EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
    3.2.1.4 | 2,4-dinitrophenyl beta-cellotrioside + H2O |
cleavage occurs between the second and third sugar residues with no release of chromophore, thus no colour change |
Cellulomonas fimi |
2,4-dinitrophenyl beta-cellobioside + D-glucose |
- |
? |
| 3.2.1.4 | 2,4-dinitrophenyl beta-D-cellobioside + H2O |
- |
Thermobifida fusca |
? |
- |
? |
| 3.2.1.4 | 2,4-dinitrophenyl beta-D-cellobioside + H2O |
- |
Thermobifida fusca Cel9A |
? |
- |
? |
| 3.2.1.4 | 2-hydroxyethyl cellulose + H2O |
- |
Geobacillus thermodenitrificans |
? |
- |
? |
| 3.2.1.4 | 2-hydroxyethyl cellulose + H2O |
- |
Geobacillus thermodenitrificans T12 |
? |
- |
? |
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O |
specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture |
Thermotoga maritima |
4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside |
- |
? |
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O |
specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture |
Trichoderma longibrachiatum |
4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside |
- |
? |
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O |
specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture |
Bacillus amyloliquefaciens |
4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside |
- |
? |
| 3.2.1.4 | 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside + H2O |
specific and sensitive assay of endo-1,4-beta-glucanase (cellulase). The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-beta-cellotrioside in the presence of thermostable beta-glucosidase. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-beta-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free D-glucose by the beta-glucosidase in the substrate mixture |
Thermotoga maritima DSM 3109 |
4'',6''-O-benzylidene beta-cellobioside + 2-chloro-4-nitrophenyl beta-D-glucoside |
- |
? |
| 3.2.1.4 | 4-methylumbelliferyl beta-cellotrioside + H2O |
- |
Thermobifida fusca |
? |
- |
? |
