EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.13.11.60 | more |
no substrate: gamma-linolenoate |
Colletotrichum graminicola M1.001 / M2 / FGSC 10212 |
? |
- |
? |
| 1.13.11.60 | more |
the dioxygenase-cytochrome P450 homologue oxidizes oleic and linoleic acids in analogy with 7,8-linoleate diol synthases (LDSs), but with the additional biosynthesis of 8,11-dihydroxylinoleic acid |
Colletotrichum graminicola M1.001 / M2 / FGSC 10212 |
? |
- |
? |
| 1.13.11.60 | more |
bifunctional enzyme, the second domain isomerizes the product (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate to (7S,8S,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate, cf. EC 5.4.4.6 |
Colletotrichum gloeosporioides KACC 40961 |
? |
- |
? |
| 1.13.11.60 | more |
the bifunctional enzyme converts linoleic acid to a product, identified as (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. The specific activity and catalytic efficiency (kcat/Km) of 7,8-diol synthase for the conversion of fatty acid to dihydroxy fatty acid follows the descending order linoleic acid, alpha-linolenic acid, oleic acid, and palmitoleic acid, indicating that the enzyme is a 7,8-linoleate diol synthase (7,8-LDS). Substrate specificity, overview. The reaction via (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) as an intermediate and the accumulation of 8-HPODE is due to a higher 8-dioxygenase activity in the N-terminal domain than hydroperoxide isomerase activity in the C-terminal domain, EC 5.4.4.6. 8-HPODE is subsequently isomerized to (9Z,12Z)-7S,8S-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) or (5R,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoic acid (5,8-DiHODE) by the C-terminal hydroperoxide isomerase domain of diol synthase. No activity with conjugated linoleic acid, docosapentaenoic acid, and docosahexaenoic acid, and no isomerase activity and no whole enzyme acitivity, but dioxygenase activity with eicosenoic acid, eicosadienoic acid, dihomo-gamma-linolenic acid, and arachidonic acid |
Colletotrichum gloeosporioides KACC 40961 |
? |
- |
? |
| 1.13.11.60 | more |
the bifunctional enzyme converts linoleic acid to a product, identified as (9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. The specific activity and catalytic efficiency (kcat/Km) of 7,8-diol synthase for the conversion of fatty acid to dihydroxy fatty acid follows the descending order linoleic acid, alpha-linolenic acid, and oleic acid, indicating that the enzyme is a 7,8-linoleate diol synthase (7,8-LDS). The reaction via (9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoic acid (8-HPODE) as an intermediate and the accumulation of 8-HPODE is due to a higher 8-dioxygenase activity in the N-terminal domain than hydroperoxide isomerase activity in the C-terminal domain, EC 5.4.4.6. 8-HPODE is subsequently isomerized to (7S,8S,9Z,12Z)-dihydroxyoctadeca-9,12-dienoic acid (7,8-DiHODE) or (5S,8R,9Z,12Z)-dihydroxyoctadeca-9,12-dienoic acid (5,8-DiHODE) by the C-terminal hydroperoxide isomerase domain of diol synthase |
Colletotrichum gloeosporioides KACC 40961 |
? |
- |
? |
| 1.13.11.60 | more |
enzyme metabolizes fatty acids to hydroperoxides with broad substrate specificity. Products show R configuration at the (n-10) positions. Fatty acids of the n-3 series are oxidized less efficiently and often to hydroperoxides with an R configuration at both (n-10) and (n-7) positions |
Fusarium oxysporum Fo5176 |
? |
- |
? |
| 1.13.11.60 | oleate + O2 |
- |
Aspergillus nidulans |
(9Z)-8-hydroperoxyoctadec-9-enoate |
- |
? |
| 1.13.11.60 | oleate + O2 |
- |
Colletotrichum gloeosporioides |
(9Z)-8-hydroperoxyoctadec-9-enoate |
- |
? |
| 1.13.11.60 | oleate + O2 |
- |
Colletotrichum gloeosporioides KACC 40961 |
(9Z)-8-hydroperoxyoctadec-9-enoate |
- |
? |
| 1.13.11.60 | oleate + O2 |
- |
Colletotrichum graminicola |
8-hydroperoxyoctadecamonoenoate + 7,8-dihydroxyoctadecamonoenoate |
- |
? |
