EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.1.1.203 | D-glucuronate + NAD+ + H2O |
inclusion of a third enzyme, Udh, apart from myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice, facilitates conversion of D-glucuronate to D-glucaric acid. Up to 1 g/l of glucaric acid is produced with the three enzymes, whereas only 0.27 g/l of glucuronic acid is produced in the system harboring the two genes of INO1 and MIOX. Udh activity is highest, more than 2fold higher than Ino1 activity and 3fold higher than MIOX activity. The high activity of Udh may pull glucose flux through the glucaric acid pathway, leading to a relatively higher titer of glucaric acid |
Pseudomonas syringae pv. tomato |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronate + NAD+ + H2O |
inclusion of a third enzyme, Udh, apart from myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice, facilitates conversion of D-glucuronate to D-glucaric acid. Up to 1 g/l of glucaric acid is produced with the three enzymes, whereas only 0.27 g/l of glucuronic acid is produced in the system harboring the two genes of INO1 and MIOX. Udh activity is highest, more than 2fold higher than Ino1 activity and 3fold higher than MIOX activity. The high activity of Udh may pull glucose flux through the glucaric acid pathway, leading to a relatively higher titer of glucaric acid |
Pseudomonas syringae pv. tomato DC3000 |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronate + NAD+ + H2O |
- |
Pseudomonas syringae pv. tomato DC3000 |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronate + NAD+ + H2O |
- |
Agrobacterium tumefaciens C58 / ATCC 33970 |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronic acid + NAD+ + H2O |
- |
Agrobacterium tumefaciens |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronic acid + NAD+ + H2O |
assay at pH 8.0 |
Pseudomonas syringae pv. tomato |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronic acid + NAD+ + H2O |
- |
Agrobacterium tumefaciens LBA4404 |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronic acid + NAD+ + H2O |
assay at pH 8.0 |
Pseudomonas syringae pv. tomato DC3000 |
D-glucaric acid + NADH + H+ |
- |
? |
1.1.1.203 | D-glucuronic acid + NADH |
- |
Penicillium camemberti |
L-gulonic acid + NAD+ |
- |
? |
1.1.1.203 | D-glucuronic acid + NADPH |
- |
Penicillium camemberti |
L-gulonic acid + NADP+ |
- |
? |