EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   6.5.1.3 | more |
RNA-stimulated exchange reaction between ATP and diphosphate |
Triticum aestivum |
? |
- |
? |
| 6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
Rnl1 has an inherent specificity for sealing tRNA with a break in the anticodon loop. The tRNA specificity is imparted by the C domain |
Tequatrovirus T4 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
| 6.5.1.3 | more |
RtcA is able of cyclizing a 2'-phosphate RNA end in high yield. The rate of 2'-phosphate cyclization by RtcA is five orders of magnitude slower than 3'-phosphate cyclization, notwithstanding that RtcA binds with similar affinity to RNA3'p and RNA2'p substrates |
Escherichia coli |
? |
- |
? |
| 6.5.1.3 | more |
synthesizes circular hammerhead ribozymes from oligonucleotides, small RNA molecules resistant to circuarization by other means can be efficiently circularized on a preparative scale using a suitable DNA template |
Tequatrovirus T4 |
? |
- |
? |
| 6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
T4 RNA ligase 1 can efficiently adenylate DNA oligos with 5' phosphate and 3' blocking group |
Escherichia coli |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
| 6.5.1.3 | more |
T4 RNA ligase possesses nick-joining activity in RNA/DNA hybrids against junctional matches/mismatches complementary to the RNA strand as a ligation template. When a junctional mismatch is present at the 30-terminal of RNA probe, the nick-joining activity of T4Rnl2 is inhibited |
Tequatrovirus T4 |
? |
- |
? |
| 6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
the best substrate is [5'-P]polyriboadenylate |
Tequatrovirus T4 |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
| 6.5.1.3 | more |
the enzyme catalyzes an adenylation reaction that is specific for ATP and either ssDNA or RNA. It does not require synthesis of a template strand for adenylation. To achieve quantitative DNA adenylation, single turnover reaction condition with substrate to enzyme ratio 1:1 should be used |
Methanothermobacter thermautotrophicus |
? |
- |
? |
| 6.5.1.3 | more |
the enzyme catalyzes formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl termini of single-stranded RNAs. It can also react with RNA with a 3'-phosphate end to generate a 2',3'-cyclic phosphate. The enzyme can additionally remove adenosine from the 3'-terminus of the RNA to produce 3'-deadenylated RNA |
Methanothermobacter thermautotrophicus |
? |
- |
? |
| 6.5.1.3 | ATP + (ribonucleotide)n + (ribonucleotide)m |
the enzyme is essential for survival of both insect and bloodstream forms of the parasite |
Trypanosoma brucei |
AMP + diphosphate + (ribonucleotide)n+m |
- |
? |
