EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
4.2.2.3 | beta-D-mannuronyl linkage in alginate |
- |
Sphingomonas sp. MJ-3 |
? |
final degradation products are alginate monosaccharides |
? |
4.2.2.3 | MG-alginate |
alginate epimerized by AlgE4 and containing 47% alpha-L-guluronic acid with no alpha-L-guluronic acid blocks, 100% activity |
Azotobacter vinelandii |
? |
isoforms AlyA1, AlyA2, and AlyA3 produce mainly DELTAG and DELTAGMG as their end products, isoform AlyA3 produces as many trimers as it does dimers and tetramers |
? |
4.2.2.3 | alginate |
isozyme A1-II performs endolytic cleavage of the glycosidic bonds, beta-elimination reaction, and shows a preference for polyguluronate instead of polymannuronate |
Sphingomonas sp. |
unsaturated algino-oligosaccharides |
isozyme A1-II produces mainly tri- and tetrasaccharides |
? |
4.2.2.3 | alginate |
- |
Sphingomonas sp. |
unsaturated alginate disaccharides + unsaturated alginate trisaccharides |
main products |
? |
4.2.2.3 | alginate |
highest activity toward poly(M)-rich substrate, moderate activity toward sodium alginate and weak activity toward poly(MG)-rich substrate, no activity toward poly (G)-rich substrate |
Aplysia kurodai |
? |
major reaction products are tri- and disaccharide along with various sizes of intermediary oligosaccharides |
? |
4.2.2.3 | more |
preferably degrades poly(M)-rich substrate producing unsaturated tri- and disaccharides and rapidly decreases the viscosity of sodium alginate solution in the initial phase of degradation |
Aplysia kurodai |
? |
major reaction products are tri- and disaccharide along with various sizes of intermediary oligosaccharides |
? |
4.2.2.3 | polymannuronate |
- |
Paradendryphiella salina |
4,5-unsaturated oligomannuronic acids |
production of a wide range of 4,5-unsaturated oligomannuronic acids, that are further degraded to unsaturated monomer and dimer as final products |
? |
4.2.2.3 | alpha-L-mannuronyl linkage in alginate |
- |
Pseudomonas sp. |
? |
products are six different di-and trisaccharides. The enzymatic hydrolysis occurs between two random guluronic acid or/and mannuronic acid residues, and produces one G residue or M residue on the reducing end and an unsaturated residue on the non-reducing end for all products |
? |
4.2.2.3 | alpha-L-mannuronyl linkage in alginate |
- |
Pseudomonas sp. HZJ 216 |
? |
products are six different di-and trisaccharides. The enzymatic hydrolysis occurs between two random guluronic acid or/and mannuronic acid residues, and produces one G residue or M residue on the reducing end and an unsaturated residue on the non-reducing end for all products |
? |
4.2.2.3 | alginate |
high affinity towards mannuronate-rich domains |
Turbo cornutus |
oligouronides |
unsaturated oligomers mostly composed of mannuronic acid as final product |
? |