EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.7.1.13 | more |
the nitrile to amine conversion proceeds through four major stages: formation of a C-S covalent bond between the substrate and the catalytic cysteine residue to form the thioimidate intermediate, hydride transfer from NADPH to the substrate to generate the thiohemiaminal intermediate, cleavage of the C-S covalent bond to generate the imine intermediate, and second hydride transfer from NADPH to the imine intermediate to generate the final amine product. The free energy barrier for the rate-limiting step, i.e. the second hydride transfer, is20.8 kcal/mol |
Vibrio cholerae O1 |
? |
- |
? |
1.7.1.13 | more |
the nitrile to amine conversion proceeds through four major stages: formation of a C-S covalent bond between the substrate and the catalytic cysteine residue to form the thioimidate intermediate, hydride transfer from NADPH to the substrate to generate the thiohemiaminal intermediate, cleavage of the C-S covalent bond to generate the imine intermediate, and second hydride transfer from NADPH to the imine intermediate to generate the final amine product. The free energy barrier for the rate-limiting step, i.e. the second hydride transfer, is20.8 kcal/mol |
Vibrio cholerae O1 ATCC 39315 |
? |
- |
? |
1.7.1.13 | more |
enzyme is highly specific for substrate preQ0 |
Pectobacterium carotovorum subsp. carotovorum PCC21 |
? |
- |
? |