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EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzaldehyde i.e. vanillin
4-hydroxy-3-methoxybenzoic acid + NADH + H+
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
-
4-hydroxybenzoic acid + NADH + H+
-
?
more
the enzyme is capable of catalysing the oxidation of a number of aromatic aldehydes, but not aliphatic aldehydes
?
-
-
more
ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues
?
-
-
more
ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues
?
-
-
aromatic aldehydes + NAD+ + H2O
-
aromatic acids + NADH
-
-
benzaldehyde + NAD+ + H2O
-
benzoic acid + NADH + H+
-
?
gentisaldehyde + NAD+
-
gentisic acid + NADH
-
-
vanillin + NAD+
-
vanillinic acid + NADH + H+
-
r
vanillin + NAD+
-
vanillinic acid + NADH + H+
-
r
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