Search Substrates and Products (Substrate)

1.2.1.29

4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O

4-hydroxy-3-methoxybenzaldehyde i.e. vanillin

4-hydroxy-3-methoxybenzoic acid + NADH + H+

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1.2.1.29

4-hydroxybenzaldehyde + NAD+ + H2O

4-hydroxybenzoic acid + NADH + H+

?

1.2.1.29

aromatic aldehydes + NAD+ + H2O

aromatic acids + NADH

1.2.1.29

benzaldehyde + NAD+ + H2O

benzoic acid + NADH + H+

?

1.2.1.29

gentisaldehyde + NAD+

gentisic acid + NADH

1.2.1.29

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the enzyme is capable of catalysing the oxidation of a number of aromatic aldehydes, but not aliphatic aldehydes

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1.2.1.29

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ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues

?

1.2.1.29

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ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues

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1.2.1.29

vanillin + NAD+

vanillinic acid + NADH + H+

r

1.2.1.29

vanillin + NAD+

vanillinic acid + NADH + H+

r