4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzaldehyde i.e. vanillin
4-hydroxy-3-methoxybenzoic acid + NADH + H+
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4-hydroxybenzaldehyde + NAD+ + H2O
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4-hydroxybenzoic acid + NADH + H+
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aromatic aldehydes + NAD+ + H2O
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aromatic acids + NADH
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benzaldehyde + NAD+ + H2O
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benzoic acid + NADH + H+
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gentisaldehyde + NAD+
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gentisic acid + NADH
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more
the enzyme is capable of catalysing the oxidation of a number of aromatic aldehydes, but not aliphatic aldehydes
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more
ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues
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more
ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues
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vanillin + NAD+
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vanillinic acid + NADH + H+
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r
vanillin + NAD+
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vanillinic acid + NADH + H+
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r