EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
   1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
the Brucella melitensis enzyme is fully active in vitro (20 mM Tris-HCl buffer, pH 8.0, with 100 mM NaCl, S-adenosyl-L-methionine (SAM), 5-aminolevulinic acid (ALA) and NADPH, aerobic, dark, 4°C) and the mononuclear non-heme iron is reducible by dithionite and then is able to react with NO as oxygen analogue in the presence of the substrate |
Brucella melitensis |
precorrin-3B + NAD+ + H2O |
- |
? |
| 1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
the Brucella melitensis enzyme is fully active in vitro (20 mM Tris-HCl buffer, pH 8.0, with 100 mM NaCl, S-adenosyl-L-methionine (SAM), 5-aminolevulinic acid (ALA) and NADPH, aerobic, dark, 4°C) and the mononuclear non-heme iron is reducible by dithionite and then is able to react with NO as oxygen analogue in the presence of the substrate |
Brucella melitensis 16M |
precorrin-3B + NAD+ + H2O |
- |
? |
| 1.14.13.83 | more |
biosynthesis of vitamin B12 |
Pseudomonas denitrificans (nom. rej.) |
? |
- |
? |
| 1.14.13.83 | more |
the enzyme is involved in the mechanism of the ring contraction process during vitamin B12 biosynthesis, comparison to the ring contraction process reaction pathway under anaerobic conditions in Propionibacterium freudenreichii ssp. shermanii, overview |
Pseudomonas denitrificans (nom. rej.) |
? |
- |
? |
| 1.14.13.83 | precorrin-3 + NADH + H+ + O2 |
- |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3x + NAD+ + H2O |
- |
ir |
| 1.14.13.83 | precorrin-3 + NADH + H+ + O2 |
biosynthesis of vitamin B12, part of the ring contractase system for oxidative ring contraction |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3x + NAD+ + H2O |
- |
ir |
| 1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
- |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
- |
? |
| 1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
- |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
- |
ir |
| 1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
- |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 |
ir |
| 1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
biosynthesis of vitamin B12 |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 |
ir |
