EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
the Brucella melitensis enzyme is fully active in vitro (20 mM Tris-HCl buffer, pH 8.0, with 100 mM NaCl, S-adenosyl-L-methionine (SAM), 5-aminolevulinic acid (ALA) and NADPH, aerobic, dark, 4°C) and the mononuclear non-heme iron is reducible by dithionite and then is able to react with NO as oxygen analogue in the presence of the substrate |
Brucella melitensis 16M |
precorrin-3B + NAD+ + H2O |
- |
? |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
- |
Pseudomonas denitrificans (nom. rej.) SC510 (RifT) |
precorrin-3B + NAD+ + H2O |
- |
ir |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 |
Pseudomonas denitrificans (nom. rej.) SC510 (RifT) |
precorrin-3B + NAD+ + H2O |
- |
ir |
1.14.13.83 | precorrin-3A + NADH + O2 |
- |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
- |
? |
1.14.13.83 | precorrin-3A + NADH + O2 |
the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview |
Rhodobacter capsulatus |
precorrin-3B + NAD+ + H2O |
- |
? |
1.14.13.83 | precorrin-3A + NADH + O2 |
the enzyme generates a hydroxy lactone intermediate |
Rhodobacter capsulatus |
precorrin-3B + NAD+ + H2O |
- |
? |
1.14.13.83 | precorrin-3A + NADH + O2 |
- |
Pseudomonas denitrificans (nom. rej.) SC510 (RifT) |
precorrin-3B + NAD+ + H2O |
- |
? |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
- |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 |
ir |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
biosynthesis of vitamin B12 |
Pseudomonas denitrificans (nom. rej.) |
precorrin-3B + NAD+ + H2O |
aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 |
ir |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 |
- |
Pseudomonas denitrificans (nom. rej.) G3575 |
precorrin-3B + NAD+ + H2O |
aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 |
ir |