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EC Number	Storage Stability
7.4.2.3	mtHsp70 and Hsp70 ATPase domain remain soluble in the absence of Hep and nucleotides when stored in Tris at pH 8.0. In the case of the ATPase domain, the protein remains soluble at concentrations as high as 0.2 mM even when stored for 24 h at room temperature. However, the mtHsp70 ATPase domain could not be stably stored at high concentrations for a similar period of time in buffers having pH values more closely resembling the physiological environment within Escherichia coli where these proteins are overexpressed, unless it is incubated with equimolar Hep

EC Number

Storage Stability

7.4.2.3

mtHsp70 and Hsp70 ATPase domain remain soluble in the absence of Hep and nucleotides when stored in Tris at pH 8.0. In the case of the ATPase domain, the protein remains soluble at concentrations as high as 0.2 mM even when stored for 24 h at room temperature. However, the mtHsp70 ATPase domain could not be stably stored at high concentrations for a similar period of time in buffers having pH values more closely resembling the physiological environment within Escherichia coli where these proteins are overexpressed, unless it is incubated with equimolar Hep

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Release 2023.1 (January 2023)