EC Number |
Storage Stability |
---|
3.5.5.5 | -20°C, in 10 mM potassium phosphate buffer, pH 7, 10 mM DTT and 50% glycerol, at least 5 months |
3.5.5.5 | -20°C, pH 7.5, 14 days, 90% loss of activity, addition of 1 M ammonium sulfate almost completely stabilizes |
3.5.5.5 | -80°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, 20% glycerol, stable |
3.5.5.5 | 4°C, 0.01 M phosphate, pH 7.5, 1 mM dithiothreitol, rapid loss of activity, presence of 20% glycerol stabilizes |
3.5.5.5 | 4°C, in 10 mM potassium phosphate buffer, pH 7, 10 mM DTT and 50% glycerol, at least 2 months, without glycerol 60% loss of activity within 1 week |
3.5.5.5 | 4°C, in the presence of 60% saturated ammonium sulfate, 2 M NaCl or 30% w/v propanediol, only about 5% loss of activity within 1 week |
3.5.5.5 | 5°C, in 0.02 M citrate buffer, pH 7-8, 10 days, about 50% loss of activity in 0.02 M Tris-HCl buffer, pH 9.3 within 10 days |
3.5.5.5 | 8°C, pH 7.5, 14 days, 75% loss of activity, addition of 1 M ammonium sulfate almost completely stabilizes |