EC Number   |
Storage Stability |
|---|
   2.1.1.165 | - 80°C, enzyme after the first gel filtration purification step, in 25 mM Tris acetate, pH 7.4, 10% glycerol, and 14 mM 2-mercaptoethanol, stable for over 2 months |
| 2.1.1.165 | -20°C to 4°C, partially purified enzyme, complete loss of activity overnight, also in the presence of protease inhibitors |
| 2.1.1.165 | -20°C, enzyme forms an aggregate with molecular mass of approximately 500000 Da |
| 2.1.1.165 | -20°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts |
| 2.1.1.165 | -20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 40% of the iodide methyltransferase activity |
| 2.1.1.165 | -20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 60% of the iodide methyltransferase activity |
| 2.1.1.165 | -20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 90% of the iodide methyltransferase activity |
| 2.1.1.165 | -80°C, after affinity chromatography, the halide/bisulfide methyltransferase becomes extremely labile losing all activity after overnight storage |
| 2.1.1.165 | 20°C, enzyme after the affinity chromatography purification step, 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 30% glycerol, 12% remaining activity after 48 h |
| 2.1.1.165 | 4°C, enzyme after anion exchange purification step, in 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 175 mM NaCl, more than 70% remaining activity after 24 h and 55% after 48 h |
