EC Number   |
Storage Stability |
|---|
    1.3.1.32 | -20°C, 100 mM Tris/HCl buffer, pH 7.0, containing 50% (vol/vol) glycerol, 1 mM dithioerythritol and 0.5 mM EDTA, purified enzyme retains 80% of its initial activity after storage for 2 months |
| 1.3.1.32 | -20°C, 30% glycerol, 4 months, enzyme retains its activity |
| 1.3.1.32 | -23°C, purified enzyme in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA after freezing, storage overnight and rethawing 35% loss of activity |
| 1.3.1.32 | -25°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several months without loss of activity |
| 1.3.1.32 | -70°C, 30% glycerol, 6 months, enzyme retains its activity |
| 1.3.1.32 | 4°C, 30% glycerol, 3 weeks, enzyme retains its activity |
| 1.3.1.32 | 4°C, purified enzyme can be stored in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA with slight loss of activity, 36% in 3 weeks |
| 1.3.1.32 | 5°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several days without loss of activity |
