EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   7.5.2.6 | -999 |
- |
kinetics of ATPase activity shown to be different from that of MsbA of Escherichia coli, colorimetric ATPase assay with purified and liposome-reconstituted protein performed, msbA mutants generated, antibiotic susceptibility test applied analyzing purified and reconstituted chimeric MsbA protein |
687605 |
| 7.5.2.6 | -999 |
- |
protein quantification by labeling of the purified native protein with MIANS, i.e. 2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid, on Cys315 |
696114 |
| 7.5.2.6 | -999 |
- |
purified MsbA is reconstituted into proteoliposomes of Escherichia coli lipid. In this system, the protein displays maximal lipid flippase activity of 7.7 nmol of lipid translocated per mg of protein over a 20 min period for (7-nitrobenz-2-oxa-1,3-diazole)-phosphatidylethanolamine (16:0, 6:0) |
711144 |
| 7.5.2.6 | 0.1445 |
- |
pH 7.5, 37°C, reconstituted recombinant His6-tagged enzyme, substrate 8 mM ATP, ATPase activity |
751020 |
| 7.5.2.6 | 0.4 |
- |
ATPase activity, purified recombinant enzyme, labeled or unlabeled with 2-(4'-maleimidylanilino)naphthalene-6-sulfonic acid |
696114 |
| 7.5.2.6 | 0.4 |
- |
purified recombinant His-tagged wild-type enzyme, ATPase activity, pH 7.5, 37°C |
749841 |
| 7.5.2.6 | 0.624 |
- |
unstimulated recombinant His6-tagged wild-type enzyme activity |
696222 |
| 7.5.2.6 | 0.726 |
- |
recombinant His6-tagged mutant S289A/S290A in presence or absence of taxol |
696222 |
| 7.5.2.6 | 0.835 |
- |
taxol stimulated recombinant His6-tagged wild-type enzyme activity |
696222 |
