EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    6.3.4.3 | -999 |
- |
- |
1506, 1528, 1529 |
| 6.3.4.3 | -999 |
- |
100.2% activity of mutant R653Q after heating at 42°C for 90 min, in 7.5% glycerol and 0.35 mM folate pentaglutamate |
698187 |
| 6.3.4.3 | -999 |
- |
102% activity of wild type enzyme after heating at 42°C for 90 min, in 7.5% glycerol and 0.35 mM folate pentaglutamate |
698187 |
| 6.3.4.3 | -999 |
- |
57.4% activity of mutant R653Q, without adding of any cofactor, after heating at 42°C for 90 min, in 7.5% glycerol |
698187 |
| 6.3.4.3 | -999 |
- |
72.3% activity of wild type enzyme, without adding of any cofactor, after heating at 42°C for 90 min, in 7.5% glycerol |
698187 |
| 6.3.4.3 | -999 |
- |
80.5% activity of mutant R653Q after heating at 42°C for 90 min, in 7.5% glycerol, 1.2 mM MgCl, 0.6 mM ATP |
698187 |
| 6.3.4.3 | -999 |
- |
88.2% activity of wild type enzyme after heating at 42°C for 90 min, in 7.5% glycerol, 1.2 mM MgCl, 0.6 mM ATP |
698187 |
| 6.3.4.3 | -999 |
- |
enzyme knock-out cells are transfected with either wild type or R653Q mutant and their ability to synthesize purines is assessed by a 14C-formate incorporation assay, the cells expressing the R653Q variant incorporate only 74% of wild-type levels of formate into DNA, the R653Q protein appears to have a significant impact on cellular nucleotide metabolism |
698187 |
| 6.3.4.3 | -999 |
- |
mitochondrial isozyme activity in different cell lines, overview |
662447 |
| 6.3.4.3 | -999 |
- |
MTHFD1L is upregulated in colon adeno-carcinomas |
701402 |
