EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
5.4.99.2 | -999 |
- |
- |
3495, 3498, 3502, 3506, 3516 |
5.4.99.2 | 0.000052 |
- |
baby hamster kidney cells grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, lacking hydroxycobalamin, 37°C, pH 7.4 |
662325 |
5.4.99.2 | 0.0002 |
- |
cell extracts preincubated with 5 microM adenosylcobalamin, 37°C, pH 7.4 |
662325 |
5.4.99.2 | 0.0021 |
- |
purified recombinant His-tagged enzyme, in presence of cofactor 5'-deoxyadenosylcobalamin, pH 7.5, 37°C |
747314 |
5.4.99.2 | 0.06 |
- |
crude homogenate, at 40°C |
701956 |
5.4.99.2 | 0.47 |
- |
- |
3504 |
5.4.99.2 | 1.33 |
- |
- |
3509 |
5.4.99.2 | 1.4 |
- |
- |
652521 |
5.4.99.2 | 2.2 |
- |
75°C, pH not specified in the publication |
726729 |
5.4.99.2 | 10.85 |
- |
after UNO Q-1 purification |
649513 |